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School of Chemical and Environmental Engineering

Now offering two distinct diplomas: Chemical Engineering and Environmental Engineering

Biological Methods for Environmental Remediation


SchoolChemical and Environmental Engineering
Course LevelUndergraduate
DirectionEnvironmental Engineering
Course IDENVE 446Semester9th
Course CategoryElective
Course ModulesInstruction Hours per WeekECTS

Lectures and Tutorials

T=2, E=1, L=0

Course TypeScientific area
Instruction/Exam LanguageGreek
The course is offered to Erasmus studentsNo
Course URLhttps//  (In Greek)



Learning Outcomes

Upon successful completion of the course, the student should:

  • Describe: Groundwater and soil remediation systems and technologies. Remediation technologies of marine environments from oil spills. Phytoremediation systems and three phase bioreactors for the treatment of contaminated soils.   
  • Distinguish: The conditions that require the application of in situ or ex situ remediation technologies.
  • Use: Computational tools for calculating the remediation time of contaminated sites.
  • Identify: The type of contamination, the easiness of degradation by biological means as well as their risks.
  • Analyze: Contaminated areas in terms of existing pollutants and alternative ways of remediating them.
  • Evaluate: The effectiveness of remediation methods.
General Competencies/Skills
  • Decision making
  • Work autonomously
  • Project design and management
  • Adaptation ability
  • Review, analyse and synthesise data and information, with the use of necessary technologies


  1. Design of in situ and ex situ remediation systems for contaminated soil and groundwater with organic compounds (chlorinated and non-chlorinated). Design of subsurface barriers (in situ reactive walls). SBR operation of bioreactors for ex situ bioremediation. Natural attenuation. Phytoremediation technologies for decontamination of soils and groundwater from heavy metals and organics. Design of air biofilters to control odors and hazardous and toxic gases. Field applications.
  2. Introduction to remediation technologies.
  3. In situ & ex situ bioremediation technologies of contaminated soil and groundwater from hydrocarbons.
  4. Design of subsurface (bio) barriers, funnel and gate systems.
  5. Design of three-phase bioreactors (slurry bioreactors). Design and operation of Sequential Batch (bio-)Reactors.
  6. Ex situ soil bioremediation with Landfarming technology.
  7. Ex situ soil bioremediation with Composting technology.
  8. Remediation of marine environments from oil spills.
  9. Natural attenuation.
  10. Phytoremediation of organic pollutants, metals/metalloids (soil & groundwater)
  11. Design of biofilters for odour control and degradation of VOCs.
  12. Biodegradation mechanisms of organic pollutants: (a) Alkanes, alkenes, aromatic hydrocarbons, PAHs.
  13. Biodegradation mechanisms of organic pollutants: (b) Chlorinated alkanes & alkenes, chlorinated aromatic hydrocarbons, explosives, pesticides.


Lecture MethodDirect (face to face)

Use of Information and Communication Technology

  • Power point presentations
  • E-class support
Instruction OrganisationActivityWorkload per Semester
- Lectures26
- Tutorials13
- Autonomous study86
Course Total125
Assessment MethodI. Written final examination: 100 % of the grade.

ΙΙ. Assignments (bonus): 10% of the grade.


  • "Biodegradation and Bioremediation”, Martin Alexander, 2nd Edition, Academic Press, London UK (1999).
  • “Bioremediation Principles”, JB Eweis, SJ Ergas, DPY Chang & ED Schroeder, McGraw-Hill, Boston MA (1998).


Course Instructor:Associate Professor A. Vlysidis (Faculty - ChEnvEng)
Lectures:Associate Professor A. Vlysidis (Faculty - ChEnvEng)
Tutorial exercises:Associate Professor A. Vlysidis (Faculty - ChEnvEng)
Laboratory Exercises: